Orientation =================== sigma_tensor ---------------- Windowsize, in which individual structure elements are calculated. Should be in the range of the underlying structure and can be optimised per setup by performing a test-series. edge ---------------- Pixel width of the edges left blank because alignment cannot be calculated there. Default is 20 px. max_dist ----------- (Optional) Specify a maximum distance around the cell center for analysis (in px). Default is None. ignore_cell_outline ----------- By default, the cell-occupied area is excluded, and matrix alignment is calculated only in the remaining area. To analyze alignment across the entire field of view (e.g., relative to the x- or y-axis), set this parameter to True. Default is False. sigma_first_blur ----------- Initial slight Gaussian blur applied to the fiber image before structure analysis. Default is 0.5. angle_sections ----------- Angle sections around the cell in degrees, used for polarity analysis of matrix fiber orientation. Default is 5°. shell_width ----------- Distance shells around the cell for analyzing intensity and orientation propagation over distance. Default is 5 µm.